human fibroblast cell line ccd 112con Search Results


ccd 112con  (ATCC)
95
ATCC ccd 112con
Ccd 112con, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccd 112con/product/ATCC
Average 95 stars, based on 1 article reviews
ccd 112con - by Bioz Stars, 2026-06
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human colonic fibroblast  (ATCC)
94
ATCC human colonic fibroblast
LINC00858 expression in CRC tumor tissues and its clinical significance. (A) Expression level of LINC00858 in CRC tissues (n=60) compared with adjacent normal tissues (n=60) was assessed using qPCR. (B) Kaplan-Meier curves for overall survival in patients with CRC with high (n=30) and low LINC00858 expression level (n=30). (C) LINC00858 expression was analyzed using reverse transcription-qPCR analysis in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic <t>fibroblast</t> (CCD-112CoN). Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; q, quantitative; WT, wild-type.
Human Colonic Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human colonic fibroblast/product/ATCC
Average 94 stars, based on 1 article reviews
human colonic fibroblast - by Bioz Stars, 2026-06
94/100 stars
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human colorectal cancer cell lines  (ATCC)
99
ATCC human colorectal cancer cell lines
LINC00858 expression in CRC tumor tissues and its clinical significance. (A) Expression level of LINC00858 in CRC tissues (n=60) compared with adjacent normal tissues (n=60) was assessed using qPCR. (B) Kaplan-Meier curves for overall survival in patients with CRC with high (n=30) and low LINC00858 expression level (n=30). (C) LINC00858 expression was analyzed using reverse transcription-qPCR analysis in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic <t>fibroblast</t> (CCD-112CoN). Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; q, quantitative; WT, wild-type.
Human Colorectal Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human colorectal cancer cell lines/product/ATCC
Average 99 stars, based on 1 article reviews
human colorectal cancer cell lines - by Bioz Stars, 2026-06
99/100 stars
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normal colon cells  (ATCC)
94
ATCC normal colon cells
LINC00858 expression in CRC tumor tissues and its clinical significance. (A) Expression level of LINC00858 in CRC tissues (n=60) compared with adjacent normal tissues (n=60) was assessed using qPCR. (B) Kaplan-Meier curves for overall survival in patients with CRC with high (n=30) and low LINC00858 expression level (n=30). (C) LINC00858 expression was analyzed using reverse transcription-qPCR analysis in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic <t>fibroblast</t> (CCD-112CoN). Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; q, quantitative; WT, wild-type.
Normal Colon Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal colon cells/product/ATCC
Average 94 stars, based on 1 article reviews
normal colon cells - by Bioz Stars, 2026-06
94/100 stars
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LINC00858 expression in CRC tumor tissues and its clinical significance. (A) Expression level of LINC00858 in CRC tissues (n=60) compared with adjacent normal tissues (n=60) was assessed using qPCR. (B) Kaplan-Meier curves for overall survival in patients with CRC with high (n=30) and low LINC00858 expression level (n=30). (C) LINC00858 expression was analyzed using reverse transcription-qPCR analysis in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN). Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; q, quantitative; WT, wild-type.

Journal: Oncology Reports

Article Title: Long non-coding RNA LINC00858 promotes TP53-wild-type colorectal cancer progression by regulating the microRNA-25-3p/SMAD7 axis

doi: 10.3892/or.2020.7506

Figure Lengend Snippet: LINC00858 expression in CRC tumor tissues and its clinical significance. (A) Expression level of LINC00858 in CRC tissues (n=60) compared with adjacent normal tissues (n=60) was assessed using qPCR. (B) Kaplan-Meier curves for overall survival in patients with CRC with high (n=30) and low LINC00858 expression level (n=30). (C) LINC00858 expression was analyzed using reverse transcription-qPCR analysis in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN). Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; q, quantitative; WT, wild-type.

Article Snippet: The human TP53-WT CRC cell lines, RKO, HCT116, Colo-678, LoVo, SW48, LS174T and human colonic fibroblast, CCD-112CoN were purchased from American Type Culture Collection ( ).

Techniques: Expressing, Reverse Transcription

miR-25-3p is a direct target of LINC00858. (A) Potential binding region between miR-25-3p and LINC00858 was predicted using StarBase V2.0 database. (B) miR-25-3p was validated as a direct target of LINC00858 using dual luciferase reporter assays in (B) SW48 and (C) RKO cells. (D) RIP assay confirmed the interaction between miR-25-3p and LINC00858. (E) The expression levels of miR-25-3p in CRC tissues (n=60) compared with that in adjacent normal tissues (n=60) were assessed using qPCR. (F) Correlation between LINC00858 and miR-25-3p expression levels was analyzed using Pearson's correlation analysis. (G) The expression levels of miR-25-3p in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN) were assessed using qPCR. (H and I) The expression levels of miR-25-3p in (H) SW48 and (I) RKO cells transfected with LINC00858 shRNAs. Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; miR, microRNA; q, quantitative; WT, wild-type; sh, short hairpin; MUT, mutant; AGO2, protein argonaute-2.

Journal: Oncology Reports

Article Title: Long non-coding RNA LINC00858 promotes TP53-wild-type colorectal cancer progression by regulating the microRNA-25-3p/SMAD7 axis

doi: 10.3892/or.2020.7506

Figure Lengend Snippet: miR-25-3p is a direct target of LINC00858. (A) Potential binding region between miR-25-3p and LINC00858 was predicted using StarBase V2.0 database. (B) miR-25-3p was validated as a direct target of LINC00858 using dual luciferase reporter assays in (B) SW48 and (C) RKO cells. (D) RIP assay confirmed the interaction between miR-25-3p and LINC00858. (E) The expression levels of miR-25-3p in CRC tissues (n=60) compared with that in adjacent normal tissues (n=60) were assessed using qPCR. (F) Correlation between LINC00858 and miR-25-3p expression levels was analyzed using Pearson's correlation analysis. (G) The expression levels of miR-25-3p in TP53-WT CRC cell lines (RKO, HCT116, Colo-678, LoVo, SW48 and LS174T) and human normal colonic fibroblast (CCD-112CoN) were assessed using qPCR. (H and I) The expression levels of miR-25-3p in (H) SW48 and (I) RKO cells transfected with LINC00858 shRNAs. Data are presented as the mean ± SEM; n≥3. *P<0.05; **P<0.01. CRC, colorectal cancer; miR, microRNA; q, quantitative; WT, wild-type; sh, short hairpin; MUT, mutant; AGO2, protein argonaute-2.

Article Snippet: The human TP53-WT CRC cell lines, RKO, HCT116, Colo-678, LoVo, SW48, LS174T and human colonic fibroblast, CCD-112CoN were purchased from American Type Culture Collection ( ).

Techniques: Binding Assay, Luciferase, Expressing, Transfection, Mutagenesis